Author(s)

Yanguo Han, Xiaoli Peng, Kai Li, Yuhetian Zhao, Yongjie Huang, Guangxin E, Yongju Zhao, Yongfu Huang

Corresponding Author

Yongfu Huang

Abstract

Our purpose was to compare the effect of KISS1-54 and KISS1-10 DNA vaccines in inhibiting gonadal growth and sexual behavior of male rats. Fifteen male rats were randomly divided into KISS1-54 (Group T1), KISS1-10 (Group T2) DNA vaccine groups and control group (Group C), in which the rats were orally given respectively KISS1-54, KISS1-10 DNA vaccines fused tPA signal peptide and the empty vector vaccine at a dose of 5 × 109 CFU/male rat at weeks 0, 3 and 6 of this study. Blood samples were collected before primary immunization and at weeks 3 and 9 after primary immunization. This study showed that both KISS1-54 and KISS1-10 DNA vaccines induced humoral immune response and the antibody titre in T1 group was significantly higher than those in T2 and C groups. Rats in T1 group has significantly lower serum kisspeptin and testosterone levels and slower growth of scrotum width than that in T2 and C groups. The frequency of mounting with females in T1 group was significantly lower than that in control group, and no significant difference was observed between T2 and C groups. The number of vaginal plugs in T1 group was obviously lower than that in T2 and C groups. These results suggest that oral KISS1-54 DNA vaccine was more effective than KISS1-10 DNA vaccine in inhibition of gonadal growth and sexual behavior in rats.

Keywords

Kisspeptin 54, Kisspeptin 10, DNA Vaccine,Testosterone Secretion, Sexual Behavior