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Web of Proceedings - Francis Academic Press
Web of Proceedings - Francis Academic Press

Effects of Noise Stress on the Pathological Sections of the Epinephelus Coioides and the Expression of Hsp70 Protein in the Liver

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DOI: 10.25236/icbsis.2019.024


Haixia Lin, Xiangwen Wang, Xingli Sun

Corresponding Author

Haixia Lin


The changes of heat shock protein Hsp70 expression in the liver of Epinephelus coioides were observed after noise stress, and the liver tissue was analyzed by pathological staining. The Epinephelus coioides with average body mass of (42.54±6.03)g was selected as the experimental object. The unmanned noise was used as the control group, and the experimental group was 130dB/1μPa, Western blot and immunohistochemistry were used respectively. The expression and localization of Hsp70 protein in liver tissues of the Epinephelus coioides at 0h, 6h, 12h, 24h, 48h, 72h and 120h after noise stress were analyzed. The results showed that the Hsp70 in the liver of the Epinephelus coioides was mainly in the cytoplasm, and its expression level showed a trend of increasing first and then decreasing, and reached the maximum after 12h of stress, and the expression level was significantly different from that before stress. However, liver tissue cells showed no obvious pathological changes such as necrosis, inflammation and fibrosis after noise stress, and the difference between the groups was not statistically significant. In summary, noise stress causes stress response in the Epinephelus coioides, and the change of immune index has a certain time effect, and the damage of its physiological function is reversible.


Epinephelus Coioides, Noise Stress, Hsp70, Western Blot, Immunohistochemistry, HE Staining