Isolation, Purification and Anticancer Effect of Hydatid
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DOI: 10.25236/iclsmh.18.016
Author(s)
Lei Zhang, Lizhong Guo, He Qian
Corresponding Author
Lei Zhang
Abstract
Hydnisella polysaccharides were extracted from the fruiting bodies of Hydrangea, and the crude polysaccharides of Hydatid were extracted by water extraction and alcohol precipitation. The purified polysaccharides were purified by DEAE-52 cellulose chromatography column. The Hep-G2 cells were cultured and the anticancer activity of the samples was studied by MTT assay. The results showed that the best inhibitory effect on Hep-G2 cells was 0.01 mg/mL boiled supernatant, the inhibition rate was 19.21%, and the positive control group was 29.56%. Therefore, Hydatid polysaccharide has a good inhibitory effect on hepatoma cell Hep-G2and plays an important role in drug selection in anticancer effects.
Keywords
Hydatid, Polysaccharide, Separation And Purification, Anticancer Effect