Author(s)

Zebin Chen, Xi Gao, Li Lin, Yuan Su, Jiani Liu, Lei Yu, Dingkang Wang, Shengguang Xu

Corresponding Author

Shengguang Xu

Abstract

The aim of this research was to clarify the effects of different inoculated pathogens on microbial species and abundances of rhizosphere soil of tobacco. The rhizosphere soil of root-knot nematode and Phytophthora parasitica infected tobacco were collected and detected. The DNA of 16S rDNA-V4 region and ITS1 region in the rhizosphere soil of infected tobacco were sequenced through high throughput sequencing technique; the abundance and diversity of microorganisms were analyzed. In the 6S rDNA-V4 region and ITS1 region, 152815 and 61505 original sequences were detected respectively. After filtering low quality sequences, 147558 and 60109 effective sequences were obtained, which could be divided into 6871 and 796 OTUs. The diversity analysis showed that pathogen inoculation caused significant differentiation in the microbial community of rhizosphere soil. The Shannon index and Chao1 index significantly increased compared with the control group, indicating that pathogen inoculation could significantly increase microbial diversity and abundance. The research on microbial diversity of rhizosphere soil of tobacco after pathogen inoculation can provide reference for the development of remediation technology on soil ecology.

Keywords

High Throughput Sequencing, Tobacco, Rhizosphere Soil, Root-Knot Nematode, Black Shank