Author(s)

Gu Ruining

Corresponding Author

Gu Ruining

Abstract

Using the elite variety "Longcheng No. 2" of Actinidia arguta as the experimental material, this study aimed to investigate the effects of different concentration combinations of naphthalene acetic acid (NAA) and 6-benzylaminopurine (6-BA) on root induction in Actinidia arguta cuttings. The goal was to screen out the optimal hormone ratio, providing theoretical basis and economic value for its tissue culture and rapid propagation. Results showed that Treatment Group I (NAA 0.10 mg•L⁻¹ + 6-BA 2.00 mg•L⁻¹) exhibited the best performance in terms of rooting rate, average number of roots, and root length. Specifically, its rooting rate reached 94.00%, with an average of 3.50 roots per cutting, an average root length of 5.60 cm, an average root diameter of 1.80 mm, and the longest main root reaching 10.5 cm, which were extremely significantly higher than those of the control group (CK) (P < 0.01). Treatment Group H (NAA 0.05 mg•L⁻¹ + 6-BA 2.00 mg•L⁻¹) achieved a rooting rate of 75.00%, with an average of 3.00 roots, an average root length of 3.10 cm, and an average root diameter of 0.90 mm. Treatment Group G (NAA 0.01 mg•L⁻¹ + 6-BA 2.00 mg•L⁻¹) showed a rooting rate of 56.30%, with an average of 3.00 roots, an average root length of 1.80 cm, and an average root diameter of 0.90 mm, both of which were significantly superior to CK (P < 0.05). Treatment Group F (NAA 0.10 mg•L⁻¹ + 6-BA 1.50 mg•L⁻¹) had the same average number of roots as Group I (3.50 roots) but with a root length of only 0.90 cm. The medium and low concentration groups (B, C, D, E) showed mediocre performance, with the average number of roots and root length mostly remaining below 1 root and 0.8 cm, respectively, with no significant differences observed. Group A (lowest concentration) had the poorest rooting effect, and the CK group failed to root throughout the experiment. In conclusion, the concentration combinations of NAA and 6-BA significantly affect the rooting of Actinidia arguta cuttings, with the ratio in Group I showing the optimal effect. This study provides theoretical basis and technical support for the rapid propagation of Actinidia arguta, and holds important practical significance for the large-scale cultivation and promotion of its elite varieties.

Keywords

Longcheng No. 2; Actinidia arguta; Plant growth hormones NAA and 6-BA; In vitro propagation; Tissue culture