Author(s)

Yifan Zhang

Corresponding Author

Yifan Zhang

Abstract

It has been proven that the CRISPR/Cas9, TALEN, and ZFN technologies were both efficient and accurate in building tumor and genetic disease models to apply gene editing for the construction of disease models and drug screening. The theoretical mechanism of human diseases can be accurately simulated and verified through experimental results on specific gene knockout or modification using CRISPR/Cas9, TALEN, and ZFN technologies. The high-throughput screening systems and gene-editing technology could be applied to rapidly screen out new particular drugs and test their preliminary therapeutic effects. It speeds up not only the new drug R&D process but also dramatically improves the efficiency and accuracy of drug screening so that it can provide technical support for personalized medicine and precision treatment. The experimental results confirmed that this gene-editing technology was efficient in constructing models for tumor and genetic diseases and further proved that it had broad application prospects in screening related drugs.

Keywords

gene editing, CRISPR/Cas9, disease model, drug screening, high-throughput screening platform