Author(s)

Min Zhang

Corresponding Author

Min Zhang

Abstract

To discuss the effect of Silibinin and VA (Cervus nippon Temminck) protein extract (VAPE) combined with 5-FU on Growth and Immune Function of Subcutaneous Transplanted tumor in SGC7901 Bearing Mice, 24 mice were inoculated subcutaneously to establish the transplanted tumor model of gastric group and Silibinin + VA+5-FU combined group, with 6 rats in each group. After the last administration, the transplanted tumor, spleen and thymus were stripped completely. The tumor inhibition rate, NK cells,T lymphocyte, Blymhocyte in peripheral were test. iNOS was detected by Western blots. The mice in the chemotherapy group gradually showed mental depression and decreased food intake and water intake with the treatment. There was no significant difference in body mass between the groups before treatment. At the end of treatment, the body mass of nude mice in the normal saline group, the chemotherapy group, the prescription group for strengthening spleen. Compared with the chemotherapy group, the body mass of nude mice in the Silibinin + VA+5-FU group was increased. Compared with 5-FU group, CD3+, CD4+,CD8+ T cell group creased in Silibinin + VA+5-FU group. Compared with 5-FU group, iNOS expression decreased in combined group. Silibinin + VA can better inhibit the growth of subcutaneous thansplanted tumor when combined with 5-FU and improve immune status.

Keywords

Silibinin, VAPE, 5-FU, Growth, Immune Functio, Transplanted tumo, SGC7901 Bearing Mice