Determination of Active Substance Content and Bacteriostasis in Polygonatum kingianum at Different Growth Ages
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Zhuqing Fan, Xiaoqian Bai
Polygonatum kingianum is an important traditional Chinese medicine. In this paper, the correlation between the exploration age and the content of polysaccharide and total flavone, and the bacteriostatic activity of the active ingredient were discussed. The findings demonstrated that in Polygonatum kingianum the maximum content of total flavonoids and polysaccharides appeared at plant growth stage of 2nd and 3rd year, respectively. While the polysaccharide, total flavone, and boiled water extract had not shown an obvious inhibitory effect on the 5 indicator bacterial and fungal strains (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candococcus albicans, and Aspergillus niger) through in-vitro bacteriostatic experiment. Therefore, it is suggested that 3-year old plant growth stage is the most suitable for harvesting, and it should be carefully used in the application and development of antibacterial properties.
Polygonatum. kingianum; different growth stages; polysaccharide; total flavonoids; water content; antibacterial activity