Isolation and Drug Sensitivity Analysis of Diarrhea Pathogens in a Duck Farm, Chongqing
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Yangyang Qiu, Muhan Xie, Ruitong Dan, Nengzhang Li, Yuanyi Peng
In order to investigate the cause of diarrhea death of young ducks in a duck farm in Tongliang, Chongqing. The pathogen was isolated and identified in various organs, blood and abdominal effusion of sick ducks, and the isolated bacteria were identified by routine bacteriological identification, 16SrDNA sequence molecular and animal pathogenicity tests, and drug resistance was analyzed by drug sensitivity test and drug resistance gene detection. The results showed that plague, Newcastle disease and Tambusu virus of duck were not detected in clinical samples, but two strains of bacteria were isolated from the organs of infected ducks, which were identified as Bacillus cereus (SPL1507) and Escherichia coli (SPL1508), respectively. Both strains of bacteria were lethal to mice, and strain SPL1508 had strong resistance. The relationship between resistance genes and phenotypes was explored through drug susceptibility test combined with drug resistance gene detection. The relationship between drug resistance genes and phenotypes was explored through drug sensitivity test combined with drug resistance gene detection. β-lactam resistance genes CTX-M gene, sulfamides sul1 and sul2 gene, and quinolone Aac(6')-Ib-cr gene were all detected, which was consistent with the results of drug sensitivity test in this study. The results provide reference for clinical disease prevention and treatment.
Duckling, diarrhea, Bacillus cereus, Escherichia coli, Genetic testing for drug resistance