Environmental Pollution Monitoring of Std Nucleic Acid Amplification Laboratory Based on Real-Time Quantitative Analysis
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Jinfeng Li, Li Zhou
PCR has developed rapidly since its inception and has important applications in various fields. Through temperature cycling in three stages of denaturation, annealing and extension, a very small amount of DNA can be amplified millions of times, which has extremely high detection sensitivity. In this paper, real-time fluorescence quantitative PCR was used to monitor the smear samples of laboratory environment and instruments and equipment. After thoroughly cleaning and disinfecting the polluted laboratory environment, instruments and equipment, etc., the test results of pollutant removal were confirmed by experiments. Except that 4 samples were not rechecked and 2 samples continued to be false negative, there were no pollution results in 36 consecutive tests. In the same period, there was no correlation between the qualified rate of environmental monitoring and the positive rate of mixed detection and split detection, which reflected that the overall environment of the nucleic acid amplification testing laboratory in our province was better. The results show that environmental monitoring of STD nucleic acid amplification laboratory is beneficial to improve the accuracy of detection results, and also protects the laboratory personnel and environment.
Real time quantitative pcr, Std nucleic acid amplification testing laboratory, Environmental pollution monitoring