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Web of Proceedings - Francis Academic Press
Web of Proceedings - Francis Academic Press

Application of Fluorescent Probes in Cell Biology

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DOI: 10.25236/icbcme.2021.035


Jiawen Lyu

Corresponding Author

Jiawen Lyu


Since the fluorescence phenomenon was first discovered, fluorescent has been wide applied in all aspects of modern society, demonstrating its increasingly important role. As a significant use of fluorescence phenomenon in practice, fluorescent probes also matter a lot in various aspects of the real society. Among all the aspects, cell biology must the one that the fluorescent probes are widely applied in. For example, the fluorescence probe can be used for effectively detecting the sensitivity and selectivity of nitroreductase (NTR) in cancer cells and imaging. Its high-efficiency observation is of great significance for people to better understand the biological function of the nitroreductase (NTR) [1]. Fluorescent probes, when detecting cells, can concurrently provide a great deal of information in vivo about intracellular ion composition, membrane potential, membrane fluidity, polarity, receptor localization, enzymes and enzyme activities. Moreover, they can realize selectable labeling of different components inside and outside cells. The example of in-vivo intracellular pH measurement illustrates the great potential of fluorescent probe technology[2]. Fluorescent probes are also one of the most powerful tools in detecting the dynamic movement of proteins. Protein-based fluorescent probes can effectively monitor the movement and interaction of cell proteins, which can deepen our understanding of protein-related mechanisms in cell biology more intuitively[3].


Fluorescent probes, Cell biology, Ntr cell information, Proteins