Author(s)

Zhang Yong, Zhao Linghua, Han Jing

Corresponding Author

Han Jing

Abstract

Objective: to investigate the mechanism of lentivirus-mediated overexpression of NR4A1 in the alleviation of lupus nephritis in lupus mice. Methods: Ten male MRL/lpr mice (LN group) and ten CS7BL mice (normal control) aged 4 months were selected. The expression level of NR4A1 in peripheral blood of two groups was compared, and the pathological changes of kidney tissue were compared. The expression level of NR4A1 in peripheral blood of LN mice with different severity of illness was compared, and the overexpression of NR4A1 inhibited cell proliferation activity. Results: Among MRL/lpr mice, type IV LN mice were the most, accounting for 7 cases, the others were type ??, type ?? and type V respectively. The renal tubulointerstitial lesions of CS7BL mice and 2 non-?? LN mice were mild. The expression of NR4A1 in peripheral blood of active LN mice was significantly lower than that of stable LN mice, and the difference was statistically significant (p < 0.05). At 48h after transfection, the proliferation activity of monocytes in transfected group was lower th

Keywords

Lentivirus mediated, NR4A1, Monocytes, Lupus nephritis