Clone the Promoter of Vvcyp86a1 Gene and Analyze Its Expression
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Yao Ge, Xinjie Zhao, Yang Zhen
The salt-induced combined promoter XM438-1 of VvCYP86A1 gene was designed, synthesized and sequence analyzed, and grape DNA was successfully extracted. After cloning the promoter of VvCYP86A1, an overexpression vector with GUS was constructed, and the verification was successful. After the transformation and identification, the results showed that salt stress could induce the gene expression driven by the promoter.