Author(s)

Yan Qi, Liu Yan, Wang Wei

Corresponding Author

Liu Yan

Abstract

Objective: To analyze the differentially expressed genes in muscle tissue of Alzheimer’s disease (AD) model mice and reveal the pathogenesis of AD from transcriptome level. Methods: The control group and AD group were set up, and the differential expression of gene transcriptomics in the model group was detected. Through functional annotation and enrichment analysis of mouse gene sequencing in the model group, genes related to the pathological mechanism of AD due to kidney deficiency and metabolic pathways were screened out. The STRING database and Cytoscape software were used for protein-protein interaction network analysis. Results: GO analysis showed that DEGs were mainly distributed in the cytoplasm, membrane and extracellular space, and could induce AD through positive/negative regulation of transcription, positive regulation of nuclear factor κB activity, and other molecular functions. The up-regulated genes of muscle tissue expression in SAMP8 mice are mainly located in the ribosome and mitochondria of cells. The main biological processes are protein synthesis, sorting and localization in cells as well as viral nucleic acid synthesis. The main molecular function is NADH reductase activity. The results of KEGG pathway enrichment analysis indicated that these genes participated in important biological pathways such as phagocytosis, lysosomes, Toll-like receptor signaling pathway, cytokine receptor interaction, and NF-κB signaling pathway. Conclusion: The AD-related differentially expressed genes provide experimental basis for AD-related mechanisms and treatment research in model mice.

Keywords

AD; Mouse muscle tissue; Gene expression difference; KEGG pathway enrichment