Relationship between CD206 expression in macrophages and pathogenesis and prognosis of Hepatocellular carcinoma
Download as PDF
Ran Fengming, Meng Qingyin
Objective: to investigate the relationship between the expression of CD206 in macrophages and the pathogenesis and prognosis of Hepatocellular carcinoma( HCC ). Methods: Human mononuclear macrophages (THP1) were cultured and stained with multi-labeling immunofluorescence staining based on tyrosine signal amplification technology using CD206 anti-proto and 4', 6- diamidino -2- phenylindomethacin (DAPI) markers simultaneously on 129 tissue microarray (TMA) containing HCC and its adjacent normal tissues. Immunohistochemistry (IHC) was used to detect the positive expression of the tumor-associated macrophage marker CD206 in HCC and the corresponding adjacent tissues on the tissue chip. Kaplan-Meier(log-rank test) and COX regression analysis were used to understand the correlation between the survival rate of patients and the expression of CD206 as well as each clinical index. Results: The number of CD68 positive macrophages (66.71±31.39) infiltrated into the carcinoma tissues was more than that of CD206 positive macrophages (30.24± 15.86) (P < 0.001), and the number of CD68 positive macrophages (79.33±22.74) in the paracancerous tissues was also more than that of CD206 positive macrophages (63.27 ±15.92) (P < 0.001). The average expression score of CD206 in carcinoma was higher than that adjacent to carcinoma (intra-carcinoma 3.68 ±1.02 points, adjacent to carcinoma 1.84 ±0.65 points, P < 0.05). The expression score of CD206 in the adjacent tissues was less than 6 points out of 84. The 5-year survival rate of HCC patients in the GP73 high expression group was not significantly different from that in the low expression group (P = 0.8127), but the survival rate of patients in the CD206 high expression group was significantly lower than that in the CD206 low expression group (P = 0.0307), and the difference was statistically significant. Conclusion: The mannose receptor CD206, which is closely related to TAM, is highly expressed in human primary hepatocellular HCC tissues as a whole, and the expression of CD206 in the adjacent tissues corresponding to each case is lower than that in the carcinoma. The high expression of TAM CD206 in cancer is an independent risk factor closely related to the prognosis of patients, which provides a theoretical basis for our subsequent intervention of CD206 in the treatment of HCC.
CD206; Macrophages; Hepatocellular HCC; Prognosis