Author(s)

Yang Hao, Guo Yunrui, Zhang Yecai, Wang Decai, Zhang Guoyun, Hou Jiali, Yang Jianming

Corresponding Author

Yang Hao

Abstract

Objective: to compare the metabolic characteristics of lung cancer cells and normal somatic cells at low temperature. Methods: The low temperature (− 5.5℃ incubator) was used to simulate the in vivo environment of the tumor, and the energy metabolism of lung cancer cell line A549 and normal somatic cells at different low temperature was compared. Results: The establishment of three-dimensional culture model in vitro was confirmed through inverted microscope, electron microscope observation and photography. N-Cadherin and catenin (α, β) are highly expressed in normal human lung cells, and AJ junction distributed on the cell membrane and its adhesion spot on the inner surface of the membrane are connected with the end of stress fiber of Actin filament assembled in the cytoplasm, suggesting the existence of N-N-Cadherin/Catenins/Actin actin molecular complex connected with membrane AJ. After low temperature, lung cancer cell line A549 has higher survival rate and smaller increase of active oxygen compared with normal body cells. Conclusion: Under low temperature stress, normal cells mainly rely on aerobic oxidation to compensate for energy, while tumor cells mainly rely on glycolysis. ROS may promote the Warburg effect and tumor tolerance to low temperature by regulating tumor cell pathways and the activities of key glycolytic enzymes.

Keywords

Lung cancer cells; Normal somatic cells; Low temperature environment; Metabolic characteristics