Effect of Exogenous Oxygen Free Radical H2O2 on SOD Activity in One-year-old Panax notoginseng Plants
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Hongping Huang, Zhiguo Shan, Chunhua Zhang
The effect of exogenous oxygen radical H2O2 on SOD activity in leaves of one-year-old Panax notoginseng seedlings under salt stress was analyzed. SOD activity is inhibited by H2O2 and decreases with the increase of the concentration of exogenous oxygen radical H2O2, resulting in the increase of superoxide radical. The one-year-old notoginseng plants were soaked in 4mmol/L oxalic acid solution for 8min as treatment and soaked in clean water as control. The plants were stored at room temperature (20℃), and the effects of H2O2 treatment on physiological indexes related to reactive oxygen species were analyzed. The results showed that exogenous H2O2 increased the activities of SOD, POD, CAT and APX enzymes, increased the contents of soluble protein, soluble sugar and proline, and decreased the contents of H2O2 and MDA in the cells under salt stress, which were significantly higher than those of the control. Exogenous oxygen radical H2O2 can enhance the ability of one-year-old notoginseng seedlings to remove ROS in vivo and reduce membrane permeability by increasing SOD activity, alleviate the inhibitory effect of salt stress on the growth of one-year-old notoginseng seedlings, and thus improve the salt tolerance of one-year-old notoginseng plants. The oxidative damage degree of plants under Ca (NO3) 2 stress alone is lower than that under NaCl stress alone..
H2O2; One-Year-Old Notoginseng Plants; Sod Enzyme Activity; Salt Stress