Study on Culture Technology of Viburnum in Vitro
Download as PDF
Hongxu Sun, Min Zhang
Inoculating M. spores in 1 / 4MS culture, 30 days can induce the formation of D0.2cm protophyte, and 120-150d can form test-tube seedlings. Protophytes of D0.5cm were taken for proliferation and culture, and the effects of proliferation were studied. The factors, the conditions for the differentiation of sporophyte seedlings and the technology of sporophyte test-tube seedlings, the results showed that: at a temperature of 25 ℃, light 12hr • d -1, light intensity 2000lx, 30g • l -1 was added to the medium , Agar 6g • l -1, pH5.8 and other conditions, the most suitable medium for protophyte proliferation is 1 / 2MS + KT5.0 mg • l -1 + IBA1.0 mg • l -1 + KH 2 PO 4 200 mg • l -1 + Activated carbon 0.5%. The protophyte that is more suitable for the differentiation of sporophyte seedlings has the characteristics of loose texture, large flakes and thick flesh. At 28 ° C, 90-100% humidity, 4 shades of yin and 6 shades of awning or under the forest, coconut velvet was selected as the substrate for qualified single plant seedlings, and the survival rate was 97.6%.
Viburnum spores protophyte proliferative sporophyte test tube seedlings