Establishment and Optimization of Extraction Method of Antimicrobial Peptide from Capsella Bursa Pastoris Seed
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Kun Li, Baozheng Shi, Weihan Wu, Rune Bai, Yonghui Li, Caiyan Lei
In order to improve the utilization rate of Capsella bursa pastoris resources, we extracted the antimicrobial peptides in shepherd's purse seed, and the analyzed the factors affecting the extraction efficiency. The results showed that the degreasing of seeds had no significant effect on the extraction rate of antimicrobial peptides; the extraction rate of peptides by ultrasonic extraction was higher, and NH4OAc was better as the extraction solution. Further experimental results showed that with the increase of the ratio of material to liquid, ultrasonic temperature and ultrasonic time, the concentration of antimicrobial peptide was higher. The best combination was dry seed powder: NH4OAc = 1:25, ultrasonic extraction at 30 ℃ for 30 minutes, and the concentration of peptide was 2.43 mg/g. By using the established method, the protein and peptide concentrations in the seeds from Beijing, Shanghai and Zhengzhou were compared. The results showed that there was little difference between the protein and antimicrobial peptide contents in the seeds from three areas. The results provide a basis for the research and application of antimicrobial peptides and the comprehensive development and utilization of shepherd's purse.
Capsella bursa pastoris, Antimicrobial peptide, Extraction efficiency, Resource development