Study on the Mechanism of Paclitaxel Induced Apoptosis of Human Hepatoma Cell Line Hepg2
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Objective: To further explore the mechanism of paclitaxel-induced apoptosis of human hepatocellular carcinoma cell line HepG2 based on previous studies. Methods: ①After paclitaxel was applied to HepG2 cells, the colorimetric method was used to determine the changes of SOD activity, MDA content, and GSH content in HepG2 cells.②The effect of paclitaxel on mitochondrial membrane potential of HepG2 cells was detected by JC-1 staining at different times. Results: ①After HepG2 cells were treated with 5, 10, 20, 40, 80 μg•L-1 paclitaxel for 48 h, the MDA content gradually increased, SOD activity and GSH content gradually decreased, and the concentration was dependent.②JC-1 staining method detected that the mitochondrial membrane potential of HepG2 cells gradually decreased after 24h, 36h, and 48h of 80μg•L-1 paclitaxel on HepG2 cells, and there was a certain time dependence. CONCLUSION: Paclitaxel disrupts the oxidative equilibrium in HepG2 cells, causes oxidative stress in normal metabolic disorders of the cells, thereby oxidatively damages cells, and induces apoptosis in HepG2 cells.
Paclitaxel, Induce, Hepatocellular carcinoma cell line hepg2, Apoptosis