Research on the Culture Technique of Multiplication and Rooting for Sorbus sibirica Tissue Culture Seedlings
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Defeng Jian, Fei Ye, Yansheng Hu
Sorbus sibirica has an important timber value, medicinal value and ornamental value, but the traditional breeding methods propagation coefficient is very low, in order to shorten the Sorbus sibirica breeding cycle in a short time and get a lot of adult, this test used in vitro reproductive technology Sorbus sibirica seedlings. Using primary culture plantlets success as a raw material, researched for multiplication phase and rooting stage, mainly to explore the best Sorbus sibirica multiplication and rooting culture training program, used five different ratios of propagation medium formulation 4 different ratios were rooting medium formulations. Sorbus sibirica multiplication and rooting plantlets were induced research aspects, the results were: MS + 6-BA 0.5 mg / L + NAA 0.12 mg / L + KT 0.2 mg / L + IBA 0.05 mg / L formula most favorable seedling propagation; 1/2MS + IBA 0.5 mg / L formula most favorable seedlings rooting rate of 100%, the average number of roots reached 4.6 and thick roots.
Sorbus sibirica; Culture medium formula; Multiplication culture; Rooting culture