Cloning and Sequence Analysis of Xyla Gene from Xylose Isomerase
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Chen Yiqiao, Zhu Wenxin, Wang Junling, Zhan Wang, Zhang Xinyue, Fan Yaxin, Yang Chuang
Xylose isomerase is an important industrial enzyme for preparing fuel ethanol and high fructose syrup. In this paper, acidophilic and thermostable microorganisms are used as xylose isomerase gene source bacteria. By means of molecular cloning and pet-28a(+) as vector plasmid, recombinant plasmid is constructed and expression strains are obtained. The result of sequencing showed that the gene was identical with the known gene in ncbi. It provides the basis for the expression and purification of recombinant protein.
Xylose Isomerase; Cloning; Vector Construction; Evolutionary Tree