Effect of Rhtgf-Β1 on Osteogenic Differentiation of Sd Rat Mscs and Its Mechanism
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in Order to Analyze the Effect of Rhtgf-Β1 on the Osteogenic Differentiation of Sd Rat Mscs, the Study of, Sd Rat Mscs Were Isolated and Purified by the Whole Bone Marrow Adherence Method, and Different Rhtgf-Β1 Were Applied by Mtt Method, and the Resulting Mscs Value-Added Activity Affects the Completion of the Test. According to the Alp (Alkaline Phosphatase) Activity and Alp Staining Positive Rate, the Optimal Concentration of Rhtgf-Β1 on Osteogenic Differentiation of Sd Rat Mscs Was Performed, and the Mscs Osteogenic Differentiation Was Performed, According to the Osteogenic Induction Fluid That Was Added, Which is Divided into 4 Groups, Normal Group, Classical Group, Rhtgf-Β1 Group, Rhtgf-Β1+ Classic Group (n Group, C Group, t Group, t+C Group). the Ability of Alp, Osteocalcin Expression, and Calcified Nodules to Differentiate into Osteogenic Differentiation Was Evaluated, and the Results Showed That Rhtgf-Β1 Was Used as the Optimal Concentration of Mscs in Sd Rats, and 5 Was the Optimal Concentration to Promote the Osteogenic Differentiation of Rat Mscs. in Addition to the Normal Three Groups, the Other Three Groups Can Promote the Osteogenic Differentiation of Mscs, and Stimulate Bmp-2 to Up-Regulate the Expression of Smad4 and Cbfal Mrna. Therefore, the Classical Group, Rhtgf-Β1, Rhtgf-Β1+ Classical Group Can Achieve Osteogenic Differentiation of Sd Rats in Sd Rats, and the Mechanism of Action is to Promote the Secretion of Bmp-2. the Tgf-Β Superfamily/Smads Signaling Pathway is Regulated. That is to Achieve Bone Differentiation.
Rhtgf-Β1; Mscs Osteogenic Differentiation; Transforming Growth Factor