Effects of Red Light Quality on Anabolism and Related Genes of Medicinal Active Components in Dendrobium Officinale Tissue Culture
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Studies have shown that red light has a significant effect on the growth and main components of Dendrobium Officinale, but the specific pathway is not clear. In order to elucidate the effect of red light on Dendrobium Officinale from the aspects of gene and metabolic pathway, the tissue culture seedlings of Dendrobium Officinale treated with red and white light were used to determine the difference of medicinal components (Dendrobium polysaccharide, mannose, Dendrobium alkaloids, etc.) under different culture conditions, and to detect the expression level of related genes. The results show that red light has some effects on the effective components of Dendrobium Officinale, especially on the synthesis and accumulation of mannose, and can promote the accumulation of alkaloids. And under different light quality, mannose consumption and transport mode will change, thus affecting the growth and development of the plant.
Dendrobium; Polysaccharide; Mannose; Alkaloid; Qpcr; Red light