Detection of Gene Expression from Vitis by Real Time Quantitative RT-PCR
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In this experiment, Vitis “shuangfeng” is used as the test materials to study gene expressed modes of 12 enzyme genes involved in the biosynthesis of phenolic compounds from skin in different growth time by real time quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the relative expression of gene was calculated by 2-∆∆Ct value. The results showed the expressed patterns of genes were different from 2 weeks to maturity after flowering. The expressed level of PAL, CHS3, F3H2, UFGT, OMT and GST showed an upward trend. The expressed level of CHI1, DFR and LDOX showed a downward trend from 2 to 8 weeks after flowering, then began upward, and the highest expression level was in maturity. The expressed level of ANR, LAR1 and LAR2 showed a downward trend to maturity in fruit growth time.
Real time quantitative RT-PCR, Vitis amurensis Rupr, gene expression.