Author(s)

Hongwei Li, Qilin Zhang

Corresponding Author

Qilin Zhang

Abstract

To screen lactic acid bacteria with bacteriostatic action from healthy chicken intestines, and determine its antibacterial effect against Staphylococcus aureus, the lactic acid bacteria against S. aureus were screened by double-layer plate method. The antibacterial effect was determined by Oxford Cup method. The active ingredient was identified by a 16s rDNA molecular marker to construct a phylogenetic tree. Eighteen lactic acid bacteria were screened from healthy chicken intestines. Two of them had good antibacterial effects against S. aureus, Salmonella choleraesuis, Escherichia coli, Shigella, Salmonella, Salmonella enteritidis, and S. Enteritidis subspecies. Two strains of lactic acid bacteria were identified as Lactobacillus plantarum and Lactococcus genus. L. plantarum strain and Lactococcus strain isolated from healthy chicken intestinal tract showed certain inhibitory effects on S. aureus and other key pathogenic bacteria.

Keywords

Lactic acid bacteria; Staphylococcus aureus; antibacterial activity