Author(s)

Fu Hong, Chen Yunlong

Corresponding Author

Fu Hong

Abstract

Astragalus membranaceus is the most commonly used material in traditional Chinese medicine. Astragaloside is an important and active ingredient. At present, the value of astragaloside has been paid more and more attention by people with the development of medical technology. Astragalus membranaceus (Astragalus membranaceus) is a dried plant root, which contains many chemical components. However, there are still many areas can be improved for the quality detection technology of astragaloside IV. Immunoblotting is a commonly used method of identification. In this paper, the concepts of immunoblotting and astragaloside IV were introduced firstly. Then the application of immunoblotting in the identification of astragaloside IV and an active ingredient of traditional Chinese medicine were analyzed from three processes: material preparation, preparation of drugs and instruments, preparation of astragaloside IV solution and identification of astragaloside IV. Finally, the application of polyacrylamide electrophoresis is helpful to reduce experimental errors and make the results more accurately. Immunoblotting assay has high feasibility for the identification of astragaloside.

Keywords

Immunoblotting; Astragaloside IV; Identification; Application