Author(s)

Shan Zhiguo, Zhang Chunhua, Qiang Jiye, Man Hongping

Corresponding Author

Zhang Chunhua

Abstract

The activities of peroxidase, polyphenol oxidase, catalase and phenylalanine ammonia lyase in tea were determined by spectrophotometer. The results showed that Xanthomonas oryzae had different degrees of infection before and after infection. There were some differences in the activities of defense enzymes between diseased and susceptible cultivars. After natural inoculation and artificial inoculation, the activities of protective enzymes of resistant and susceptible varieties increased rapidly. The increase of protective enzymes of resistant varieties was significantly greater than that of susceptible varieties, but there was no significant difference in the activities of CAT enzymes. The changes of POD and PPO isozyme bands in tea plants were studied by polyacrylamide gel electrophoresis. The results showed that the two isozyme bands had the same characteristics before and after artificial inoculation. There are some differences in resistance and sensory changes. Before inoculation with Xanthomonas oryzae. The POD isozymes of resistant and susceptible cultivars had 4 to 5 bands, and PPO isozymes had 3 to 4 bands. The susceptible cultivars were darker in color. It was thicker, while resistant varieties had lighter and better color. After artificial inoculation, the number of POD and PPO isozyme bands of resistant and susceptible varieties increased to 5-6 and 4-5, respectively. The new enzyme bands of diseased tea varieties appeared earlier than those of susceptible tea varieties, and the enzyme bands were thicker, darker and more active, which indicated that the isozymes of different resistant tea varieties were different.

Keywords

Tea Moire Leaf Blight; Physicochemical Components of Tea; Effects