Isolation of Tea Brown Pigment from Pu'er Tea and Its Balance of Human Metabolic Function
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DOI: 10.25236/medsbe.2019.008
Author(s)
Shan Zhiguo, Zhang Chunhua, Qiang Jiye, Man Hongping
Corresponding Author
Zhang Chunhua
Abstract
Pu 'er tea is a kind of post-fermentation tea with various health functions, of which polyphenol and theabrownin are important functional components. Pu 'er tea theabrownin is a kind of polyphenol oxidation product with very complex structure and belongs to natural pigment. Analysis bags with different molecular entrapments can effectively separate theabrownins. AFM showed that the morphologies of theabrownin particles with different molecular weights were not uniform, and the single molecule was in island state or granular aggregate structure. When the particles gather more, they are linearly linked and have more branches or form a network structure.The macroporous resin was used to separate the tea brown pigment into two components with different polarities. The DEAE-52 anion exchange resin was used to separate the components with higher polarity and low phenolic content according to the difference of ionic strength. Several different properties of the tea brown fraction. Different components of the tea brown pigment have a certain difference in total sugar, total phenol and protein content. The four major components of TBsP1 isolated have similar composition and different contents, which provide reference for studying the composition, structure and function of the tea brown pigments.
Keywords
Pu'er Tea; Theophyllin; Separation; Human Metabolism