Author(s)

Junling Wang, Chuang Yang, Jinghui Yu, Yawei Xu, Ming Yu, Fuli Tang

Corresponding Author

Junling Wang

Abstract

In this experiment, γ-aminobutyric acid was produced by lactic acid bacteria fermentation with germinated brown rice powder as culture medium. Sodium glutamate and vitamin B6 were added to the fermentation process to increase the yield of GABA. Through single factor experiment and orthogonal experiment, the optimum conditions were obtained as follows: the concentration of vitamin B6 was 0.3 mmol/L, the concentration of sodium glutamate was 0.6 mg / mL, the culture time was 72 h, and the culture temperature was 30 ℃. The yield of GABA was 7.83 mmol / L. This study has opened up a new way for the preparation of γ-aminobutyric acid.

Keywords

Brown Rice, Lactic Acid Bacteria, Γ-Aminobutyric Acid, Orthogonal Test