Author(s)

Song Jin, Xi Gao, Zebin Chen, Yuan Su, Jiani Liu, Lei Yu, Dingkang Wang, Shengguang Xu

Corresponding Author

Shengguang Xu

Abstract

With Yunyan 87 as the research material, this paper used Illumina MiSeq high throughput sequencing technology to study the effect of marigold cultivation on microbial diversity of tobacco planting soil under two kinds of planting patterns. The results showed that the diversity of bacterial Alpha was represented as bacterial abundance CC3.2 > AB3.2 > BB3.2 and bacterial diversity BB3.2 > AB3.2 > CC3.2. The diversity of fungal Alpha was represented as fungi abundance BB3.2 > AB3.2 > CC3.2 and fungi diversity BB3.2＞AB3.2＞CC3.2. At the level of genus, the dominant bacteria of three samples were similar; three kinds of bacteria with higher abundances were Kaistorbacte, Marinobacter and Nitrososphaera. There were only small differences in the abundances of the same genus. The content of Nitrososphaera in CC3.2 was lower than that in AB3.2 and BB3.2 by 0.80% to 0.91%. The three samples had similar dominant fungi, but the abundances of fungi varied greatly. After the planting of marigold, the abundance of Aspergillus increased from the original number of 0.89% to values between 5.61% and 7.70%. Compared with single cropping of tobacco, marigold inter planting with tobacco increased the abundance by 8.6 times. The abundance of Sordariales sp rose from the original number of 3.75% to values between 4.09% and 5.03% after marigold cultivation. The abundance of Corynascella was between 6.24% and 7.39% under monoculture, and sharply decreased to 0.04% under the condition of tobacco inter planting with marigold. The decrease rate was over 99%.

Keywords

High-Throughput Sequencing, Marigold Cultivation, Tobacco-Planting Soil, Microorganism, Diversity