Author(s)

Zebin Chen, Xi Gao, Li Lin, Yuan Su, Jiani Liu, Lei Yu, Dingkang Wang, Shengguang Xu

Corresponding Author

Shengguang Xu

Abstract

This paper aims to analyze microbial diversity of tobacco rhizosphere soil at different growth stages. With tobacco rhizosphere soil as the research object, high throughout sequencing was carried out on DNA of 16S rDNA-V4 region and fungi of ITS1 region in rhizosphere soil of tobacco. Soil samples were collected at different growth stages. Qiime and Mothur software were used to collate and count Operational Taxonomic Unit (OTU) in samples; the abundances and Alpha diversities of species were analyzed. The results showed that the order of bacterial diversity was squaring period> decaying period > resettling stage > mature period > vigorous growing period. The diversity of fungi was highest at the mature period, and showed declining trend. At the level of genus, the most dominant bacteria at resettling stage were Pseudomonas, accounting for 2.70%; the most dominant bacteria at vigorous growing period were Alcanivorax, accounting for 15.55%; the most dominant bacteria at mature period were Candidatus Nitrososphaera, accounting for 20.16%; the most dominant bacteria at decaying period were Streptomyces, accounting for 5.16%; the most dominant bacteria at squaring period were Marinobacter, accounting for 14.24%. The diversity analysis showed that dominant bacteria in rhizosphere soil varied greatly at different growth stages. The diversities and abundances of bacteria at the mature, vigorous growing and squaring periods were higher than those of resettling and decaying periods. The most dominant fungi at resettling period were Aspergillus, accounting for 12.19%; the most dominant fungi at vigorous growing period were Mortierella, accounting for 4.29%; the most dominant fungi at mature period were Un--s-Fungi sp, accounting for 2.91%; the most dominant fungi at decaying period were Chaetomium, accounting for 3.87%; the most dominant fungi at squaring period were Un--s-Tremellomycetes sp, accounting for 10.24%. The diversity analysis showed that dominant fungi in rhizosphere soil varied greatly at different growth stages. The species diversities and total abundances of fungi at the resettling period were higher than those of squaring, decaying, mature and vigorous growing periods. Fungal diversity also declined with the passage of time.

Keywords

High Throughput Sequencing, Growth Period, Tobacco, Rhizosphere, Soil Microorganism.